Cleaning solution and method of cleaning laboratory consumables

ABSTRACT

A cleaning solution for cleaning laboratory consumables comprises sodium percarbonate in a concentration of from 0.9% w/w to 1.6% w/w based on a total weight of the solution and at least one diluent to 100% w/w.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part and claims priority to U.S.patent application Ser. No. 14/266,330, filed Apr. 30, 2014, which inturn claims the benefit of U.S. Provisional Patent Application Ser. No.61/817,715, filed Apr. 30, 2013 and U.S. Provisional Patent ApplicationSer. No. 61/890,523, filed Oct. 14, 2013, each of which is herebyincorporated by reference in its entirety.

FIELD OF THE INVENTION

The present technology relates to a washing device and a method ofwashing laboratory consumables, and more particularly to a pipette tipwashing device and a method of washing pipette tips.

BACKGROUND

Every year around 4,000,000 pounds of plastic pipette tips, after asingle use, are disposed of in landfills globally, leading tosignificant environmental pollution and costs. A typical laboratoryconsumes several thousand pipette tips daily for samples and assayprocedures. Due to the lack of options for cleaning plastic consumables,the labs discard pipette tips after each use. Such high consumption ofplastic tips adds $25,000-$1.5M to the annual operation cost to each ofthe approximately 14,000 research laboratories in the US.

Devices that are capable of efficient pipette tip cleaning andsterilization could save businesses substantial amounts of money intheir scientific operations and drastically reduce the amount of wasteproduced in the course of operations. Few devices have been developedfor this purpose to date. In some cases, laboratories have developedsmall-scale cleaning methods to reuse a few pipette tips, such as single96-tip cases. In some small-scale automatic liquid handling instruments,there are setups for the cleaning of tips with solutions. Neither ofthese options, however, is large enough in scale to be useful in a largeindustrial, government, or academic laboratory that may use hundreds ofpipette tips every day. Additionally, labs must have absolute confidencethat a cleaning system has completely removed all contaminants from thepipette tips so that there is no carryover, a term for the contaminationpresented into an experiment by equipment used in a prior experiment.

Thus, there is a need for a large-scale and economical method for thecomprehensive cleaning and sterilization

BRIEF SUMMARY

In one embodiment of the invention, a cleaning solution for cleaninglaboratory consumables (such as pipette tips) comprises sodiumpercarbonate in a concentration of from about 0.9% w/w to about 1.6% w/wbased on a total weight of the solution, and at least one diluent to100% w/w. The sodium percarbonate may be in a concentration of 1.2% w/wbased on the total weight of the solution. The at least one diluent maycomprise deionized water.

In addition to the cleaning solution, as described above, other aspectsof the present invention are directed to corresponding methods forcleaning laboratory consumables.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWING(S)

The foregoing summary, as well as the following detailed description ofthe disclosure, will be better understood when read in conjunction withthe appended drawings. For the purpose of illustrating the disclosure,there are shown in the drawings embodiments which are presentlypreferred. It should be understood, however, that the disclosure is notlimited to the precise arrangements and instrumentalities shown. In thedrawings:

FIGS. 1A and 1B are exploded perspective views of an exemplaryembodiment of a pipette tip washing device of the present disclosure.

FIG. 2 is a perspective view of the exemplary pipette tip washing deviceof the present disclosure.

FIGS. 3A and 3B are front and rear perspective views of the exemplarypipette tip washing device of the present disclosure with the topcompartment in an open position.

FIG. 4A is a perspective view of a top compartment of the pipette tipwashing device illustrated in FIG. 1.

FIG. 4B is a perspective view of a receiving compartment of the topcompartment illustrated in FIG. 4A.

FIG. 5A-5D are an exploded perspective view, a top perspective view, aside view, and a bottom view, respectively, of the top compartment ofthe pipette tip washing device and manifold dispensers configured to belocated in the top compartment.

FIG. 6A is an exploded perspective view of a manifold dispenser of thepresent invention.

FIG. 6B is a top perspective view of the manifold dispenser of thepresent invention.

FIG. 6C an exploded view of a manifold dispenser of the presentinvention.

FIG. 6D is a bottom perspective view of the manifold dispenser of thepresent invention.

FIG. 6E is a magnified view of portion A of the manifold dispenserillustrated in FIG. 6D.

FIGS. 7A and 7B are an exploded perspective view and an exploded sideview of another embodiment of the manifold dispenser of the presentinvention.

FIGS. 8A and 8B are partial phantom front and perspective views ofanother exemplary embodiment of a pipette tip washing device of thepresent disclosure.

FIG. 8C is a side view of the pipette tip washing device illustrated inFIGS. 8A and 8B in an opened position.

FIGS. 9A-9D are perspective views of the pipette tip washing deviceillustrated in FIGS. 8A-8C in various positions during operation of thedevice in accordance with the present disclosure.

DETAILED DESCRIPTION

An exemplary pipette tip washing device 10 is illustrated in FIGS. 1-3B.The pipette tip washing device 10 includes a top compartment 12,manifold dispensers 14, tip racks 16, a tip rack support 18, a middlecompartment 20, a wash sleeve 22, a bottom compartment 24, anultraviolet (UV) light source 26, and optional transducers 27, althoughthe pipette tip washing device 10 may include other elements in otherconfigurations. This exemplary technology includes a number ofadvantages including providing a device and method for the efficient andeconomical sterilization of a large number of pipette tips for use inlarge scale laboratory settings.

Referring more specifically to FIGS. 4A-4B, an exemplary top compartment12 of the exemplary pipette tip washing device 10 is shown. The topcompartment 12 includes a front cover 30 removably coupled to areceiving chamber 32, although the top compartment 12 may include otherelements in other configurations. Front cover 30 is constructed ofstainless steel, although front cover may be constructed of othernumbers and types of materials. Front cover 30 is removably coupled tothe receiving chamber 32 through one or more one or more screws 34 whichare inserted into holes 36 in the receiving chamber 32, although otherattachment methods may be utilized to removably couple front cover 30 toreceiving chamber 32.

Receiving chamber 32 is configured to receive the one or more manifolddispensers 14 as illustrated in FIGS. 5A-5D. In this example, receivingchamber 32 is configured to receive four manifold dispensers 14,although receiving chamber 32 may receive other numbers of manifolddispensers 14 in other configurations. In this example, the receivingchamber 32 includes internal rails 38 on the sidewalls which support themanifold dispensers 14 and one or more holes 40 disposed on the topsurface of the receiving chamber 32 configured to receive screws forsecure attachment to the manifold dispensers 14, although the receivingchamber 32 may include other elements in other locations to support andsecurely attach the manifold dispensers 14 within the receiving chamber32.

Referring again to FIGS. 1A and 1B, the receiving chamber 32 furtherincludes one or more input ports 42 configured to receive liquid inputfittings 44 a and 44 b, which are disposed on opposite sides of the wallof the receiving chamber 32 and are configured to receive liquid intothe pipette tip washing device 10 from an external control source (notshown). The external control source may include a pump, control valves,and electronics necessary to deliver wash liquids to the pipette tipwashing device 10 in accordance with the present technology. Thereceiving chamber 32 is constructed of stainless steel, although thereceiving chamber 32 may be constructed of other numbers and typesmaterials.

Referring now to FIGS. 6A-6E, an exemplary manifold dispenser 14 isshown. The manifold dispenser is configured to be inserted intoreceiving compartment 32. The manifold dispenser 14 includes a manifoldadapter 46 which may be coupled to a manifold body portion 48, and ano-ring adapter 50, although the manifold dispenser 14 may include otherelements in other configurations. In this exemplary manifold dispenser14, the manifold adapter 46 is coupled to the manifold body portion 48by screws 52 which are configured to be inserted into the holes 54 and56 in the manifold adapter 46 and the manifold body portion 48,respectively, although other attachment mechanisms may be utilized. Theo-ring adapter 50 is located at the contact point between the manifoldadapter 46 and the manifold body portion 48 to provide a watertight sealwhen the manifold adapter 46 and the manifold body portion 48 arecoupled together.

The manifold adapter 46 includes a liquid input 58 configured to receivea manifold elbow input 60 which may be coupled to the liquid inputfittings 44 b, as illustrated in FIG. 1, to transfer liquid into themanifold adapter 46, although other liquid input ports in otherconfigurations may be utilized to introduce liquid into the manifoldadapter 46. The manifold adapter 46 also includes exit ports 62, asillustrated in FIG. 6C, which are coupled to the manifold body portion48 to transfer liquid from the manifold adapter 46 to the manifold bodyportion 48. The manifold adapter 46 is constructed of a chemicalresistant material such as, by way of example only, polyphenylenesulfide or polyetheretherketone (PEEK), although the manifold adapter 46may be constructed of other types and numbers of materials.

The manifold body portion 48 includes input ports 64 which areconfigured to be aligned with the exit ports 62 of the manifold adapter46 when the manifold adapter 46 and the manifold body portion 48 arecoupled. The input ports 64 receive liquid transferred from the manifoldadapter 46. The manifold body portion 48 also includes a plurality ofliquid outputs 66 configured to operably direct fluid introduced intothe manifold dispenser 14 from the manifold body portion 48 into to thewash sleeve 22 to contact a number of laboratory consumables, such aspipette tips. In this example, the liquid outputs 66 are nozzlesextending from the manifold body portion 48, although other types ofliquid outputs may be utilized in other configurations. The number ofliquid outputs 66 in the manifold body portion 48 may match the numberof pipette tips in the tip rack 16, which are held below the manifoldbody portion 48, as illustrated in FIG. 1. By way of example, themanifold body portion 48 may include 96 liquid outputs 66, althoughmanifold body portion 48 may include other numbers of liquid outputs 66configured for use with different tip racks 16.

Manifold body portion 48 may further optionally include a number ofholes 67 in the surface opposite to the liquid outputs 66, the holes 67being configured to receive fiber optic needles 68. The fiber opticneedles 68 extend through the manifold body portion 48 and extend intoliquid outputs 66 to direct light into the wash sleeve 22. In oneexample, the fiber optic needles 68 are attached to the UV-light source26 to direct UV-light to the wash sleeve 22, although the fiber opticneedles 68 may receive light from other light sources in otherconfigurations. The manifold body portion 48 further includes a topsupport structure 69 including a hole 70 configured to be aligned withhole 40 in the receiving chamber 32. Screw 72 may be inserted throughhole 40 in the receiving chamber 32 and hole 70 in the top supportstructure 68 to securely attach manifold body portion 48 to thereceiving chamber 32, although other attachment mechanisms may beutilized. The manifold body portion 48 is constructed of a chemicalresistant material such as, by way of example only, polyphenylenesulfide or polyetheretherketone (PEEK), although the manifold bodyportion 48 may be constructed of other types and numbers of materials.

FIGS. 7A and 7B illustrate another exemplary manifold dispenser 114 thatmay be utilized with the pipette tip washing device of the presentdisclosure. Manifold dispenser 114 is the same in structure andoperation as the manifold dispenser 14 illustrated in FIGS. 6A-6E,except as illustrated and described herein. Like parts will be describedusing like reference numerals. The manifold dispenser 114 includes amanifold adapter 146 which may be coupled to a manifold body portion148, and an o-ring adapter 50, although the manifold dispenser 114 mayinclude other elements in other configurations. In this exemplarymanifold dispenser 114, the manifold adapter 146 is coupled to themanifold body portion 148 by screws 52, although other attachmentmechanisms may be utilized. The o-ring adapter 50 is located at thecontact point between the manifold adapter 146 and the manifold bodyportion 148 to provide a seal when the manifold adapter 146 and themanifold body portion 148 are coupled together.

The manifold adapter 146 includes a liquid input 58 configured toreceive a manifold elbow input 60 which may be coupled to the liquidinput fittings 44 b, as illustrated in FIG. 1, to transfer liquid intothe manifold adapter 146, although other liquid input ports in otherconfigurations may be utilized to introduce liquid into the manifoldadapter 146. The manifold adapter 146 also includes a single exit port162, which is coupled to the manifold body portion 148 to transferliquid from the manifold adapter 146 to the manifold body portion 148.The manifold adapter 146 is constructed of a chemical resistant materialsuch as, by way of example only, polyphenylene sulfide orpolyetheretherketone (PEEK), although the manifold adapter 146 may beconstructed of other types and numbers of materials.

The manifold body portion 148 includes an input port 164 which isconfigured to be aligned with the exit port 162 of the manifold adapter146 when the manifold adapter 146 and the manifold body portion 148 arecoupled. The input port 164 receives liquid transferred from themanifold adapter 146. The manifold body portion 148 also includes aplurality of liquid outputs 66 configured to operably direct fluidintroduced into the manifold dispenser 114 from the manifold bodyportion 148 into to the wash sleeve 22 to contact a number of laboratoryconsumables, such as pipette tips. In this example, the liquid outputs66 are nozzles extending from the manifold body portion 148, althoughother types of liquid outputs may be utilized in other configurations.The number of liquid outputs 66 in the manifold body portion 148 maymatch the number of pipette tips in the tip rack 16, which are heldbelow the manifold body portion 148, as illustrated in FIG. 1. By way ofexample, the manifold body portion 148 may include 96 liquid outputs 66,although manifold body portion 148 may include other numbers of liquidoutputs 66 configured for use with different tip racks 16.

Manifold body portion 148 may further optionally include a number ofholes 67 in the surface opposite to the liquid outputs 66, the holes 67being configured to receive fiber optic needles 68. The fiber opticneedles 68 extend through the manifold body portion 148 and extend intoliquid outputs 66 to direct light into the wash sleeve 22. In oneexample, the fiber optic needles 68 are attached to the UV-light source26 to direct UV-light to the wash sleeve 22, although the fiber opticneedles 68 may receive light from other light sources in otherconfigurations. In this embodiment, the manifold body portion 148further includes holes 170 configured to receive springs 171. Thesprings 171 are configured to receive shoulder bolts 172 to attach themanifold body portion 148 to the receiving compartment 32, althoughother attachment mechanisms may be utilized. The springs 171 allow arange of movement of manifold body portion 148 to provide alignment withthe pipette tips prior to washing. The manifold body portion 148 isconstructed of a chemical resistant material such as, by way of exampleonly, polyphenylene sulfide or polyetheretherketone (PEEK), although themanifold body portion 48 may be constructed of other types and numbersof materials.

Referring again to FIGS. 1A and 1B, the pipette tip washing device 10includes four tip racks 16 configured to be inserted into tip racksupports 18, although the pipette tip washing device 10 may includeother numbers of tip racks. Although the wash device 10 is describedwith respect to pipette tips, it is to be understood that the presentinvention could be utilized with racks which hold other types oflaboratory consumables, such as laboratory consumables with similarconfigurations to pipette tips. Further, the present technology may beutilized with pipette tips of various sizes. By way of example 1, 2, 8,16 or more tip racks 16 may be utilized for maximization of thethroughput in the space available in the receiving compartment 32. Inthis example, the tip racks 16 hold 96 pipette tips for a total of 384pipette tips in the pipette tip washing device, although the tip racks16 may hold other numbers of pipette tips, or other laboratoryconsumables. The tip rack supports 18 support the tip racks 16 above thewash sleeve 22.

The middle compartment 20 of pipette tip washing device 10 is configuredto be attached to the top compartment 12 through hinges 74 such that thetop compartment 12 may be lifted in order to insert tip racks 16 intotip rack support 18, although other attachment mechanisms may beutilized to attach the middle compartment 20 to the top compartment 12.The middle compartment 20 is capable of receiving fluid output by theone or more manifold dispensers 14 such that fluid does not enter thebottom compartment 24.

The middle compartment 20 further includes a floor 76 comprising amaterial transparent to ultraviolet (UV) light, such as by way ofexample only, quartz, although other transparent materials may beutilized. Floor 76 is configured to provide a water tight-seal thatprevents fluid introduced into the middle compartment 20 from enteringthe bottom compartment 24. Drain 78 exits the floor 76 and directs fluidthrough drain fitting 80. Drain fitting 80 is coupled to a waste drainelbow fitting 82. The waste drain elbow fitting 82 extends from thedrain fitting 80 from the bottom of the middle compartment 20 into thebottom compartment 24 and is coupled to waste output fitting 84 whichexits the pipette tip washing device 10 through the bottom compartment24, although the drain may have other configurations. The middlecompartment is further configured to receive the wash sleeve 22. Thesidewalls of the middle compartment 20 are constructed of stainlesssteel, although the middle compartment 20 may be constructed of othernumbers and types of materials.

The wash sleeve 22 or wash chamber is configured to be inserted into themiddle compartment 20. The wash sleeve 22 is constructed of a materialcapable of reflecting at least a portion of the UV-light from theUV-light source 26, although the wash sleeve 22 may be constructed ofother types and numbers of materials. In another embodiment, the washsleeve 22 may be constructed of a transparent material, such as quartzby way of example only, in order to direct UV-light into the middlecompartment 20 from other light sources, such as the light sourcesillustrated in FIGS. 8A and 8B below. The wash sleeve 22 is replaceableand protects the sidewalls of the middle compartment 20 from fluid.

The bottom compartment 24 is located below the middle compartment 20 andseparated by floor 76. The bottom compartment 24 houses UV-light source26, such as a UV lamp, which is configured to direct UV-light throughthe floor 76 into the middle compartment 20, although the UV-lightcompartment may include other numbers and types of light sources inother configurations. The bottom compartment 24 further may include oneor more transducers 27 to direct sound in the ultrasonic range into themiddle compartment 20, although other devices may be utilized to directsound in other ranges to the middle compartment 20. The bottomcompartment is easily accessible to replace the UV-light source 26. Thebottom compartment 24 is protected from fluids by the floor 76. Thebottom compartment 24 further includes an exit port 86 located underdrain 78 in floor 76. The drain elbow fitting 82 extends from the drainfitting 80 from the bottom of the middle compartment 20 into the bottomcompartment 24 and is coupled to waste output fitting 84 which exits thepipette tip washing device 10 through exit port 86 in the bottomcompartment 24, although the drain may have other configurations.

Another embodiment of an exemplary pipette tip washing device 110 isillustrated in FIGS. 8A-9D. Pipette tip washing device 110 is the samein structure and operation as the pipette tip washing device 10illustrated in FIGS. 1-3B, except as illustrated and described herein.Like parts will be described using like reference numerals. In thisembodiment, top compartment 12 is coupled to middle compartment 20 byrails 190 that allow top compartment 12 to be raised vertically alongthe rails 190 above middle compartment 20. In one embodiment, topcompartment 12 is raised in an automated process by mechanical cylinder192 which is coupled to rod 194, as shown in FIG. 8C, although topcompartment 12 may be manually raised.

In this embodiment, middle compartment 20 is disposed on telescopicguides 196 that allow the middle compartment 20 to be opened as a drawerfor insertion of tip racks 16, although other devices that allow middlecompartment to be opened may be utilized. Middle compartment 20 may beopened and closed in an automated process by cylinder 198, althoughmiddle compartment 20 may be opened manually as well. Middle compartment20 further includes UV light sources 126 disposed on the inside surfacethereof. The UV light sources 126 may be utilized with a transparentwash sleeve to direct light onto pipette tips held in pipette tip racks16 during operating of the pipette tip washing device 110.

In this embodiment, the middle compartment 20 further includesmechanical cylinders 199 that may operatively raise and lower the tipracks 16 when the top compartment 12 is in an open position. Themechanical cylinders 199 may be utilized to agitate the pipette tips toimprove wash quality during the wash process or to assist in the dryingprocess.

Referring now to FIGS. 9A-9D, the pipette tip washing device 110 isillustrated in various states of operation. FIG. 9A shows the pipettetip washing device 110 in an opened position with top compartment 12raised and middle compartment 20 opened as a drawer for the insertion ofpipette tips. FIG. 9B shows the pipette tip washing device 110 in theclosed position for washing the pipette tips. FIGS. 9C and 9D illustratethe movement of the tip racks 16 with the top compartment 12 raised inorder to agitate the pipette tips to assist in the drying process.

A method for washing pipette tips using the pipette tip washing device10 will be described with reference to FIGS. 1-9D. One or more tip racks16 containing pipette tips are loaded into the pipette washing device10, although the present method may be utilized for other laboratoryconsumables. The one or more tip racks 16 are supported in the pipettetip washing device 10 by tip rack support 18. The one or more tip racks16 may hold 24, 48, 96, 384, or 1536 pipette tips, by way of example,although the one or more tip racks 16 may hold other numbers of pipettetips. The pipette tip washing device 10 may be utilized with pipettetips with volumes of 10 μL-5 mL, such as 10 μL, 50 μL, 1 mL, or 5 mLpipettes, by way of example, with corresponding lengths between 30mm-120 mm, although the pipette tip washing device 10 may be utilizedwith pipette tips with other sizes and configurations. Pipette tipwashing device 10 may be utilized to clean both conductive andnon-conductive pipette tips. Manifold body portions 48 having differentnumbers of liquid outputs 66 may be interchanged based on the number ofpipette tips in the one or more tip racks 16.

One or more liquid washing or rinsing solutions are directed into thepipette tip washing device 10 through input ports 42. The liquidsolutions may be pumped into the pipette tip washing device 10 by thepump in the external control source (not shown). In one embodiment, theliquid may be provided in a pressurized flow to assist in the cleaningprocess. The liquid solutions may be deionized water, bleach, hydrogenperoxide, one or more enzyme solutions, ethanol, detergent, purifiedwater, water, ammonia, isopropanol, alcohol, a solution capable ofsubstantially rinsing or decontaminating plastic, or combinationsthereof, although other liquid solutions in other combinations may beutilized. In one example, the following liquid solutions are applied forwashing/rinsing prior to draining: a) deionized water, b) deionizedwater and 5-10% bleach solution, c) deionized water, d) ethanol,although other liquid solutions may be applied in other combinations.

In one embodiment of the invention, one of the cleaning solutions mayinclude a mixture of sodium percarbonate and a diluent. Sodiumpercarbonate (Na₂CO₃.1.5H₂O₂) is an adduct of sodium carbonate andhydrogen peroxide. Sodium percarbonate with 13-14% active oxygen may beused. The diluent may comprise deionized water, purified water, or thelike, or any other suitable diluent. The sodium percarbonate cleaningsolution may include one or more other active or inactive ingredients(in addition to the diluent), or may exclude any other ingredients.

Sodium percarbonate is a powder that is mixed in the diluent to form thedesired cleaning solution. In one specific embodiment of the invention,25 grams (g) of sodium percarbonate is mixed in 2 liters (L) ofdeionized water, such that the resulting solution has a concentration ofsodium percarbonate of 1.2% w/w. In alternative embodiments, thecleaning solution may have a concentration of sodium percarbonate ofbetween about 0.9% w/w (e.g., 17.5 g of sodium percarbonate in 2 L ofwater) to 1.6% w/w (e.g., 32.5 g of sodium percarbonate in 2 L ofwater). The sodium percarbonate powder is added to the diluent, and thenthe combination is mixed via any suitable means (e.g., shakingvigorously or via stir bar) to suitably dissolve the sodium percarbonatepowder in the diluent.

The sodium percarbonate cleaning solution may be used to cleaninglaboratory consumables, such as pipette tips, in a washing device ofembodiments of the claimed invention. The sodium percarbonate cleaningsolution may be used as the sole cleaning solution (although one or morerinsing liquids or solutions may be used), or the sodium percarbonatecleaning solution may be used in conjunction with one or more othercleaning solutions.

The liquid solutions enter the manifold dispensers 14 through the liquidinput 58 in the manifold adapter 46. The liquid solutions are thendirected through the manifold body portion 48. The liquid solutions exitthe manifold body portion 48 at liquid outputs 66 and enter the middlecompartment 20 to contact the pipette tips held in the one or more tipracks 16. The liquid outputs 66 may direct the liquid solution throughthe pipette tips or may possibly direct the pipette tips to uptakewashing fluid for washing/rinsing.

The liquid solutions enter the middle compartment 20. Quartz floor 76prevents the liquid solution from entering the bottom compartment 24.The liquid solution may exit the pipette washing device 10 through drain78. In one example, the pipette tips may be submerged in the liquidsolutions that are filled on top of the quartz floor 76 prior to beingremoved from the middle compartment 20 through drain 78.

The liquid solutions are then removed from the pipette washing device 10through drain 78. In one example, 4 cycles of liquid solutions (waterrinse, soap rinse, etc.) are directed into the middle compartment 20prior to draining the fluids. After draining the liquid solutions, thepipette tips are at least substantially dried, although the pipette tipsmay be completely dried. In one example, the pipette tips aresubstantially dried by agitating the pipette tips, although other dryingmechanisms may be utilized to substantially dry the pipette tips.

Throughout the wash process, the UV light source 26, by way of example,is engaged to expose the outer surfaces of the pipettes to ultravioletlight to sterilize the pipettes. The UV light source 26 directs light tothe pipettes through the quartz floor 76, although UV light may bedirected from other directions from other UV light sources, such aslight sources 126 as illustrated in FIGS. 8A and 8B. The UV light source26 may further direct light through the fiber optic needles 68 locatedin the openings of the liquid outputs 66 in the manifold body portion48. The fiber optic needles 66 direct UV light to the inside of thepipette tips to provide sterilization of the interior surfaces of thepipette tips. Additionally, the pipette tips may be exposed to sound inan ultrasonic range from the one or more transducers 27, which directthe sound in the ultrasonic range into the washing chamber.

Having thus described the basic concept of the invention, it will berather apparent to those skilled in the art that the foregoing detaileddisclosure is intended to be presented by way of example only, and isnot limiting. Various alterations, improvements, and modifications willoccur and are intended to those skilled in the art, though not expresslystated herein. These alterations, improvements, and modifications areintended to be suggested hereby, and are within the spirit and scope ofthe invention. Additionally, the recited order of processing elements orsequences, or the use of numbers, letters, or other designationstherefore, is not intended to limit the claimed processes to any orderexcept as may be specified in the claims. Accordingly, the invention islimited only by the following claims and equivalents thereto.

That which is claimed:
 1. A method for washing laboratory consumables,comprising: directing one or more liquid solutions to contact aplurality of laboratory consumables held by a rack, the directingcomprising introducing the one or more liquid solutions into a fluidinput and out of a plurality of fluid outputs of a manifold; anddraining the one or more liquid solutions via a waste drain disposedproximate to a washing chamber configured to receive the one or moredirected liquid solutions following the contact by the one or moreliquid solutions with the plurality of laboratory consumables;substantially drying the plurality of laboratory consumables; wherein atleast one of the one or more liquid solutions comprises a mixture ofsodium percarbonate in a concentration of from 0.9% w/w to 1.6% w/wbased on a total weight of the solution and at least one diluent to 100%w/w.
 2. The method of claim 1, where the sodium percarbonate is in aconcentration of 1.2% w/w based on the total weight of the solution. 3.The method of claim 1, wherein the at least one diluent comprisesdeionized water.
 4. The method of claim 1, further comprisingsubstantially submerging the plurality of laboratory consumables in atleast one of the one or more liquid solutions prior to draining the oneor more liquid solutions from the waste drain.
 5. The method of claim 1,wherein the one or more liquid cleaning solutions further comprise oneor more of deionized water, bleach, hydrogen peroxide, one or moreenzyme solutions, ethanol, detergent, purified water, water, ammonia,isopropanol, alcohol, a solution capable of substantially rinsing ordecontaminating plastic, or combinations thereof.
 6. The method of claim1, further comprising: exposing the plurality of laboratory consumablesto light in an ultraviolet (UV) range, the exposing comprisingilluminating a light source to direct the light in the UV range throughone or more of a UV transparent floor or one or more walls of thewashing chamber.
 7. The method of claim 6, wherein the directing furthercomprising at least one of washing or rinsing the plurality oflaboratory consumables with at least two liquid solutions prior toexposing the plurality of laboratory consumables to the light in the UVrange.
 8. The method of claim 6, wherein the exposing further comprisesexposing at least an interior portion of the each of the plurality oflaboratory consumables to the light in the UV range via a plurality offiber optic channels extending from the manifold into the plurality offluid outputs.
 9. The method of claim 6, wherein the exposing furthercomprises introducing the light in the UV range into the plurality offiber optic channels directly from the light source.
 10. The method ofclaim 6, wherein the exposing comprises exposing both an interior and anexterior portion of each of the plurality of laboratory consumables tothe light in the UV range.
 11. The method of claim 1, wherein thedirecting further comprises directing the one or more liquid solutionsto contact a plurality of laboratory consumables held by a plurality ofracks and wherein the plurality of laboratory consumables comprisepipette tips.
 12. The method of claim 1, further comprising exposing theplurality of laboratory consumables to sound in an ultrasonic range, theexposing comprising energizing one or more transducers to direct thesound in the ultrasonic range into the washing chamber.
 13. The methodof claim 1, further comprising agitating the plurality of laboratoryconsumables.
 14. The method of claim 1, wherein a number of theplurality of fluid outputs of the manifold equals a number of theplurality of laboratory consumables such that the one or more liquidsolutions directed from each of the plurality of fluid outputs contactsis directed at a separate one of the plurality of laboratoryconsumables.
 15. The method of claim 1, further comprising: lowering themanifold vertically relative to the plurality of laboratory consumablesheld by the rack; and raising the manifold vertically relative to theplurality of laboratory consumables held by the rack.
 16. The method ofclaim 1, wherein the plurality of laboratory consumables comprises aplurality of pipette tips.